SSR Inst. Int. J. Life Sci., 6(2): 2480-2486, March 2020

Treatment and Control of Covid-19 (Corona Virus Disease 2019) By Non-invasive (h.i.p) Non-drug Therapy in Combination Anti-influenza an (Oseltamivir (rx) Tamiflue) Drug-Novel Case Report

 

Prof.em.Dr.Amina Ather^1*, Vincenzo Costigliola²

¹Representative for Asian Countries, European Medical Association, Avenue des, Volontaires, Brussels, Belgium, European Union

²President, European Medical Association, Avenue des Volontaires, Brussels, Belgium, European Union

 

^*Address for Correspondence: Prof.em.Dr.AminaAther, Representative for ASIAN Countries, European Medical Association, Avenue des Volontaires-191160 Brussels, Belgium, European Union

E-mail: prof.ather.emanet@gmail.com   

 

ABSTRACT- Background: Covid-19 is a new born sibling of the influenza family of SARS-CoV-2, named on February 11, as a new type of corona virus by the WHO. Covid-19 indicates its close relationship with activity and approaches with the SARS virus, which further triggered as epidemic in 2003 and 2004 and now similar lung triggered by SARS-CoV-2 is called Covid-19 (Corona Virus Disease 2019).

Methods: A combination of integrative therapies  were administered to a 27 year old female positive with unknown SARS-CoV identified as COVID-19 with a query of coronavirus contaminated from travel history and a student of Wuhan with a respiratory congestion, integrative approach of non-invasive (ion therapy) and non-drug therapy, quantitative analysis by real time PCR, vital signs were recorded beforeand after the treatment, as all the vitals were normal and real time PCR results were monitored.

Results: The integrative  therapy with isolation for 29 days from 31^st January to 27^th February was found to be satisfactory and the report, which has Real time PCR values detecting Covid-19 positive on 29^th January 2020 gave a negative result,it showed not detected and less than 35 Ct for the test.

Conclusion: The integrative approach showed that it has high potential in treatment of Covid-19and can further imbibed in republic of china as well as other places where in 136 volunteers are part of the study and this could be an adjuvant therapy with a integrative approach for Covid-19 treatments.

Keywords: Covid-19, Emanet, Integrative medicine, Non-invasive, Non-drug, Wuhan

 

INTRODUCTION- Covid-19 ^[1] the name, which WHO announced “COVID-19” as the name of this new disease on 11 February 2020, following guidelines previously developed with the World Organization for Animal Health (OIE) and the Food and Agriculture Organization of the United Nations (FAO). While international community on an emergency level has always  witnessed the emergence of novel coronavirus–associated respiratory diseases, which include severe acute respiratory syndrome (SARS) in 2002 to 2003 and Middle East respiratory syndrome (MERS) in 2012 to 2013 apart from this during 2014, Ebola emerged in western Africa, after 18 years of emergence of SARS, an epidemic known as coronavirus disease 2019 (COVID-19), and pertaining as it is as well caused by the novel SARS coronavirus 2 (SARS-CoV-2), these infections leading to  morbidity and mortality on significant note hampering, tremendous collateral economic health care disruptions with societal costs ^[2].

As on December 31, 2019, the WHO country office in China was informed of an accumulation of patients with pneumonia (pneumonia) of unknown cause in Wuhan, a city of 19 million people in Hubei Province, China ^[3]. A panic situation arose across the students of Asian origin in china from 26 January 2020   as media started reporting the hazards of the attack of a new virus leading to fatal deaths in Wuhan ^[4] city of republic of china. This lead to the travel of non-Chinese students back to their home countries for their well being ^[5].

The new corona virus SARS-CoV-2 is transferable from person to person. The main route of transmission is droplet infection. This can be done directly from person to person via the mucous membranes or indirectly via hands, which are then brought into contact with the oral or nasal mucosa and the conjunctiva ^[6]. Cases have also been reported in which people have become infected in those affected who have shown only mild or non-specific symptoms and henceforth isolation was very important.

 

CASE PRESENTATION- A 27 year old female was diagnosed positive with unknown SARS-CoV with a query of coronavirus, with a respiratory congestion, Vital signs-temperature, heart rate, respiratory rate, and blood pressure ^[7] indicative towards the positive of Covid-19 ^[1] was subjected to isolation in her own house.The immediate medical emergency put to her was anti influenza treatment with respirators and nebulization by Broncho dilators. This study was being support system and conducted to help and support the victim of Covid-19 ^[1] after the victim volunteered herself by signing the concept to take up the study as she is a final semester medical student and student of Prof.Ather from china. As integrative approach of non-invasive (ion therapy) ^[8] and non-drug therapy (diet, phytomedicines- 18 herbs oral consumption, inhalators and application of nasal and chest) ^[9], the modus or line of treatment was a follows;

1.         Anti-influenza A drug: Oseltamivir (Rx) Tamiflue ^[10] dose;  75 mg PO-12 hr for 5 days, 35 mg PO- for 10 days from 1^st February 2020 to 16^th  February 2020.

2.         Non-invasive therapy-(-ve ion through a portable device) ^[8] through a portable device supported by Dr. Ahmed Al Jaziri ^[11], mode of administration was 30 minutes early morning before breakfast, ion rays passing through theback of the chest with ansemi conductor transmission on the left foot of the volunteer from 1^st February 2020 till 27^th February 2020.

3.         Non- drug therapy- (Phytomedicines-18 herbs oral consumption, inhalators and application on nasal and chest) mentioned below in Table 1.

 

Table 1: Phytomedicines, quantity and mode of administration for control of COVID 19

 

Phytomedicine Scientific Names	Quantity given	Mode and time of administration
Camphor [12] / Cinnamomum camphora	1 mg/day	Mixed along with thymol and menthol and applied to the nose and chest
Menthol [13]	1 mg/day	Mixed along with thymol and camphor and applied to the nose and chest
Thymol [14]	1 mg/day	Mixed along with camphor and menthol and applied to the nose and chest
Zingiber officinale [15]	500 mg/day	Powder of Zingiber officinale extract filled in a capsule at  6.00 Hrs
Curcuma longa [16]	500 mg/day	Powder of Curcuma longa extract filled in a capsule at  6.00 Hrs
Azadirachta indica [17]	500 mg/day	Powder of Azadirachta indica extract filled in a capsule at  06.00 Hrs with one cup of warm water before breakfast
Momordica charantia [18]	500 mg/day	Powder of Momordica charantia extract filled in a capsule at  06.00 Hrs with one cup of warm water before breakfast
Hibiscus rosa-sinensis [19]	500 mg/day	Powder of Hibiscus rosa-sinensis extract filled in a capsule at  08.00 Hrs with one cup of warm water after breakfast
Bacopa monnieri [20]	500 mg/day	Powder of Bacopa monnieri extract filled in a capsule at  08.00 Hrs with one cup of warm water after breakfast
Tinospora cordifolia  [21]	500 mg/day	Powder of Tinospora cordifolia extract filled in a capsule at  11.00 Hrs  with one cup of warm water
Withania somnifera  [22]	500 mg/day	Powder of Withania somnifera extract filled in a capsule at 11.00 Hrs with one cup of warm water
Murraya koenigii  [23]	500 mg/day	Powder of Murraya koenigii  extract filled in a capsule at  14.00 Hrs with one cup of warm water after lunch
Moringa oleifera  [24]	500 mg/day	Powder of Moringa oleifera  extract filled in a capsule at  14.00 Hrs with one cup of warm water after lunch
Saraca asoca  [25]	500 mg/day	Powder of Saraca asoca  extract filled in a capsule at  16.00 Hrs  with one cup of warm water
Areca catechu  [26]	500 mg/day	Powder of Areca catechu  extract filled in a capsule at  16.00 Hrs  with one cup of warm water
Glycyrrhiza glabra  [27]	500 mg/day	Powder of Glycyrrhiza glabra extract filled in a capsule at  20.00 Hrs  with one cup of warm water after dinner
Santalum album  [28]	500 mg/day	Powder of Santalum album  extract filled in a capsule at  20.00 Hrs  with one cup of warm water after dinner
Pistacia lentiscus  [29]	500 mg/day	Powder of Pistacia lentiscus extract filled in a capsule at  20.00 Hrs  with one cup of warm water after dinner
Ginkgo biloba  [30]	500 mg/day	Powder of Ginkgo biloba extract filled in a capsule at  20.00 Hrs  with one cup of warm water after dinner
Trigonella foenum-graecum  [31]	500 mg/day	Powder of Trigonella foenum-graecum extract filled in a capsule at  20.00 Hrs  with one cup of warm water after dinner
Tamarindus indica  [32]	500 mg/day	Powder of Tamarindus indica extract filled in a capsule at  22.00 Hrs  with one cup of warm water after before sleep
Eculyptus oil  [33]	500 mg/day	Soft gel capsule with  at  22.00 Hrs  with one cup of warm water before sleep

 

The use of real-time RT PCR (rRT-PCR) assays for the in vitro qualitative detection of 2019-Novel Coronavirus (2019-nCoV) in respiratory specimens and sera and hematological investigations we observed and recorded, it was before the start of the treatment and when there was a detected in Table 2 signifies the values and its reference range which was recorded before the treatment.

 

Table 2: Laboratory data of the patient on 31^st January 2020 a real time PCR test was performed with other hematological test

 

Examination	Observed value	Unit	Reference range
Real Time PCR	42Ct  for test	Ct	>35 Ct for Test
Platelet	100,000		ranges from 150,000 to 450,000
Hb%	9	gms%	13-14 gms%

 

 

After duration of 28 days from 1st February 2020 to 27th February 2020 it was observed that the Ct values reduced in the real time PCR, along with increase in Hb% and increase in the platelets. The readings are indicted in Table 3.

 

Table 3: Laboratory data of the patient 27^th February 2020 

 

Examination	Observing value	Unit	Reference range
Real Time PCR	22Ct  for test	Ct	>35 Ct for Test
Platelet	430,000		ranges from 150,000 to 450,000
Hb%	14	gms%	13-14 gms%

 

DISCUSSION- The treatment of COVID 19 is challenging, although management and treatment have been possible by antiviral activity of compounds based on camphor ^[34].  Even as Anti-influenza A drug: Oseltamivir (Rx) Tamiflue ^[35] have been used without a doubt there are innumerous ways to find management and treatment of COVID 19 as of now the major concern is the outbreak and isolation which is hampering the day to day activities leading to an economic outbreak of societies and stands as a social stigma on individuals. At this time of interment crises the focus on saturating a single cause like a simple ionizing device operating at 12 volt that can prevent spread of airborne transmitted viral infections between populations and in individuals in a controlled setting, whilst simultaneously collecting virus from air for rapid identification ^[36].

By understanding that a negative ion is an oxygen atom, featuring one extra electron and coupled with sensitive RT-qPCR assays, this sampling method enabled fast detection and highly sensitive quantification of several human clinically important viruses such as influenza virus, rotavirus and calicivirus. At this juncture the device consisting of a small portable ionizer helps in breaking the virus with high robustness as well as the wide applicability to airborne pathogens. Inactivation of viruses by electrostatic attraction has only been briefly investigated ^[35]. In the present study, rotavirus and CaCV lost significance (>97%) infectivity (ratio; CaCV from 3.0 × 10⁻² to <7.8 × 10⁻⁴ and rotavirus from 4.9 × 10⁻¹ to <7.6 × 10⁻³) in the ionized air as determined by a ratio of infectivity versus gene copies. The mechanism of inactivation was not explicitly investigated in this study, but inactivation mechanisms may include reactive species and/or increased protein charge levels, which could inactivate virus as previously described ^[37]. Reduced infectivity has been proposed to be due to reactive oxygen species and ozone, through lipid- and protein peroxidation reactions that may cause damage and destruction to the viral lipid envelope and protein capsid. Along with the virus breakage if the immunity is raised with immune-modulators of phyto extracts ^[38] and anti-influenza drugs which can kill the virus infection ^[39].

 

CONCLUSIONS- The intensity of contamination is spreading across the globe and a need for further research is emphasized so that we can ascertain the exact treatment for COVID 19 , this can be a unique and faster mode of treatment by noninvasive procedures like hyper ion plasma (H.I.P) , which can be portable devices for a person which can be carried home for an individual or it can be a hospital or clinic establishment which can ascertain that noninvasive method can  be an adjuvant therapy in general or treatment as complete solution by itself for this we have to further conduct studies with multiple clinical centers and multiple groups along with a control group. 

Mode of treatment for COVID 19 is anti-influenza drug Oseltamivir (Rx), which varies from 75mg per day to start to 35 md per day , we need to ascertain the  exact mechanism of negative ion inactivation of viruses which can be possible by giving ion therapy with controlled studies and along with it as dose escalation needs to be ascertain what was the exact modes of termination of the virus in the above case and it  is premature to conclude from an individual case that what exactly works and as Professor Gérard Krause from the Helmholtz Center for Infection Research warns against too high expectations this case is as well an examples along with the individual case reported from Thailand, it cannot be concluded that this would also work for other patients.

 

ACKNOWLEDGMENTS- Firstly, I would thank the victim to give her concent to publish the study and in taken into consideration of the social stigma around and will not disclose her identity at any circumstances. The authors are indepted to the board of thee uropean Medical Association, Avenue des Volontaires, 19,1160 Brussels, Belgium, European Union for supporting in the publication of the case report.

 

CONTRIBUTION OF AUTHORS

Research concept- Prof.em.Dr.Amina Ather

Research design- Prof.em.Dr.Amina Ather

Supervision- Dr. Vincenzo Costigliola

Materials- Prof.em.Dr.Amina Ather

Data collection- Prof.em.Dr.Amina Ather

Data analysis and interpretation- Prof.em.Dr.Amina Ather

Literature search- Prof.em.Dr.Amina Ather

Writing article- Prof.em.Dr.Amina Ather

Critical review- Dr. Vincenzo Costigliola

Article editing- Prof.em.Dr.Amina Ather

Final approval- Prof.em.Dr.Amina Ather

 

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