Reseach Article (Open access) |
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Int. J. Life. Sci. Scienti.
Res., 4(6):
2059-2065, November 2018
Study on the Guard Hair of Some
Domesticated Ungulate Species of the Saurashtra Region of the Gujarat, India
Vibhuti B. Raval1*, Bhupat B.
Radadia1, Ashvinkumar H. Italiya2
1Department
of Biology, Shree M. & N. Virani Science College, Saurashtra University, Rajkot,
Gujarat, India
2Biology Department, Regional
Forensic Science Laboratory, Junagadh, Gujarat, India
*Address for
Correspondence: Ms. Vibhuti B. Raval, Research Scholar, Department
of Biology, Shree M. & N. Virani Science College, Saurashtra University,
Rajkot, Gujarat, India
ABSTRACT- The
study was carried out to recognize the domesticated species belonging to the
family Bovidae by their specific macro-microscopic features of dorsal guard
hair characteristics. Nowadays the domesticated animals played a vital role in
the dairy industry and in providing easy prey-base for the various top
predators which found to occur throughout the Gujarat area including protected
and non-protected areas. In this, we collected control hair samples from the
various cattle owners distributed in whole Saurashtra region of the Gujarat
State in the year 2018. The total randomly picked up one hundred twenty guard
hairs from a dorsal region of the four different cattle species were analyzed
under microscopes to avail authenticated and the photographic evidence for the
further carnivore scat analysis through this key. In this study, we used the
recognizable qualitative and quantitative features of cuticle as well as
medulla of the hair. Medullary Index
(MI) found higher in domesticated Sheep 0.93±0.01, followed by Water Buffalo 0.9±0.02, followed by domesticated Goat
0.77±0.01, which further followed by domesticated
Cow 0.5±0.10 µm with lower MI. In
this study we used the identifiable qualitative and quantitative features of
Cuticle as well as Medulla.
Key words:
Guard Hair, Ungulates, Domesticated Bovid, Protected Areas, Predatory
Carnivore, Qualitative and Quantitative
INTRODUCTION
Mammal hairs play a significant role in
thermoregulation, body shape maintenance, waterproofing and protection from the
variety of pollution. The scientific study of the hair is called as trichology
and this field originated in the mid-1800s. In fact, identification of mammal hair
was earlier performed by [1]. There are mainly two types of
mammalian hair found: Guard hairs that are usually thick and bristly and fine
hairs which are curled and thin comparatively [2].
Hair
analyses through their morphological cuticular scale and medullary features
have been widely used to distinguish among mammalian species of interest
largely in the different field of Science. The domesticated ungulates comprise
the order Artiodactyla (even-toed) and family Bovidae.
It
was one of old but become advanced with the latest instrument and technology of
this modern era. It is the best effective techniques widely used in the
forensic as well as in the field of ecology. The distinctive MI for each
species is very much suitable for species confirmation and useful to identify
unknown hair samples as well as for the study of the feeding ecology of large
carnivore animals by their scat analysis within and around the protected areas.
The scale count and scale pattern also found to be species-specific and therefore
beneficial to identify the hair of unknown species with the help of all
external and internal morphological features of the hair [3].
MATERIALS
AND METHODS- In existing study, guard hairs of the
four different domesticated bovine animals were thoroughly evaluated in the
RFSL, during the year of 2018. The guard hairs were selected from animal
species belonging to the family Bovidae with different genus (Jafrabadi Water
Buffalo, Gir Cow, Gohilwadi Goat, and Patanvadi Sheep). For almost a
century, compound and comparison microscopes remained the only reliable tools
for the microscopic identification of characteristics found in animal hair [4].
All the control guard hairs were
collected separately and labeled further from the different cattle owners.
After collection of hairs of each species were properly washed in running water
for few times to remove dirt. Accordingly total one hundred twenty hairs of
four domesticated bovine species were cleaned and degreased in acetone. Then
randomly picked up thirty hairs from each species were examined. First, each
hair was embedded in Gelatin layered microscopic slide (or Nail paint) for
scale imprinting. Then it was analyzed for microscopic features using ZEISS,
Fluorescence light microscope. The microscope has inbuilt measurement software
with the camera attachment namely: Axiocam Imager. First studied under 10X
view, then in 40X view for detailed morphometric study.
The morphological external features of
the hair shaft were recorded with suitable details and measurement. Later
internal morphological features of the hair medulla were studied previously and
recorded separately in the data book. On the basis of all external and internal
morphological as well as quantitative features of the guard hair, each species confirmation was made. To distinguish
the species, several differencing aspects of hair were to be considered to
achieve the confidence limit.
RESULTS-
Various
morphological and quantitative features of the guard Hair of four domesticated
species of family Bovidae studied in detail. The mean value as well as standard
deviation regarding various features of hair shaft and medulla are given in
below Tables.
Table 1: Systematic
position and conservation status of four domesticated cattle
S. No. |
Species |
Order |
Family |
Genus |
1 |
Gir cow |
Artiodactyla |
Bovidae |
Bos |
2 |
Jafrabadi water buffalo |
Artiodactyla |
Bovidae |
Bubalus |
3 |
Gohilwadi goat |
Artiodactyla |
Bovidae |
Apra |
4 |
Patanvadi sheep |
Artiodactyla |
Bovidae |
Ovis |
Table 2: Different factors of Hair
of the four domesticated ungulate species of Saurashtra
S. No. |
Species |
Mean length of hair (cm) |
Average diameter of hair shaft (µm) |
Average diameter of medulla (µm) |
Medullary Index (µm) |
1 |
Gir cow |
1.11 |
19.75 |
10.97 |
0.5 ±0.10 |
2 |
Jafrabadi buffalo |
7.63 |
51.6 |
45.9 |
0.9±0.02 |
3 |
Gohilwadi goat |
2.33 |
45.01 |
34.66 |
0.77±0.01 |
4 |
Patanvadi sheep |
4.13 |
36.93 |
19.4 |
0.93±0.01 |
Different
Medullary Index (MI) of the four domesticated ungulate species along with the
standard error given (Fig. 1).
Fig. 1: Medullary Index (Mean±SE) of hairs domesticated ungulates
of Saurashtra
Various
morphological and quantitative features of the guard Hair of four domesticated
species of family Bovidae studied in detail. The mean value, as well as
standard deviation regarding various features of hair shaft and medulla are given
in Table 3 (Fig. 5).
Table 3: Morphological features of
Hair of the four domesticate Bovine species
S. No. |
Species |
Medulla pattern |
Scale pattern |
Color of hair |
Hair surface |
1 |
Gir cow |
Simple medulla (Amorphous) |
Regular & irregular wave |
White, brown /black |
Smooth |
2 |
Jafrabadi buffalo |
Simple medulla (Amorphous) |
Streaked |
Black |
Rough |
3 |
Gohilwadi goat |
Narrow medulla |
Regular & irregular wave |
White, brown /black |
Smooth |
4 |
Patanvadi sheep |
Wide medulla |
Regular wave |
White & brown |
Smooth |
Fig.
2: Scale and Medulla pattern of the hair of Gir cow
Fig. 3: Scale and Medulla patterb of the hair of Jafrabadi
buffalo
Fig. 4: Scale and Medulla pattern
of the hair of Gohilwadi goat
Fig.
5: Scale and Medulla pattern of the hair of Patanvadi sheep
DISCUSSION
Systematic position and
conservation status- All the four domesticated species
belonging to the single order Artiodactyla and similar family Bovidae. All four
domesticated cattle species acquire different genus and species. The Gir Cow
belongs to genus Bos and Jafrabadi Water Buffalo belong to genus Bubalus,
while Gohilwadi Goat belongs to Apra genus and Patanvadi Sheep
belong to genus Ovis respectively. Further randomly picked thirty dorsal
guard hairs of each species thoroughly evaluated under the microscope (Table
1).
Mean
value of MI, Shaft, Medulla and whole hair- The medullary
index is the specialized feature of the hair. Every species of the mammals can
be identified on the basis of their medullary features (Table 2, Fig. 1). Maximum MI found in Patanvadi Sheep (0.93±0.01 µm), followed by Jafrabadi Water Buffalo (0.9±0.02 µm), followed by Gohilwadi Goat (0.77±0.01
µm), which further
followed by Gir Cow (0.5±0.10 µm) as lowest
comparatively. MI
is the specialized feature of the guard hair. Each and every species of the
mammals can be identified on the basis of their medullary index (Table 2, Fig.
1).
Morphological
features of the hair- There are a number of important
morphological features to be considered for the identification and confirmation
of the hair for an unknown species. The Gir cow and Jafrabadi Buffalo
have simple type of medulla whereas Gohilwadi Goat has narrow medulla and
Patanvadi Sheep has wide medulla comparatively (Table 3, Fig. 2, 3, 4 & 5).
The scale pattern of the Gir Cow
and Gohilwadi Goat found to be similar but Patanvadi Sheep has regular wave
pattern instead. The Jafrabadi buffalo has unique streaked scale pattern which
is completely distinguishing it from other three domesticated species [4].
CONCLUSIONS- On
the basis of morphological features like hair color, texture, Cuticle scale
type and medulla type along with the several measuring features of the hair
such as medulla diameter, shaft diameter and MI to identify, and confirm the
hair of the species from four different domesticated bovine species. Mostly
from the above four bovids, Jafrabadi Water buffalo has streaked scale pattern
with Simple and wide medulla, while Gir Cow and Gohilwadi Goat shows similar
Regular and irregular wave pattern. In Patanvadi Sheep has regular wave pattern
of the scale with wide medulla. Whereas Gir Cow has Simple amorphous medulla
and Gohilwadi Goat shows narrow medulla type. On another hand the quantitative
features of the guard hair of animal species are distinctive and
species-specific. On the basis of medulla type, only water buffalo shows
distinctive scale type out of four species of domesticated bovine. The maximum
MI percentage found in Patanvadi Sheep, followed by Jafrabadi Water Buffalo,
followed by Gohilwadi Goat, followed by Gir Cow with lowest comparatively. The quantitative features of the guard hair
of animal species are distinctive and species-specific. These differential features of the
hair are largely suitable and authenticated, hence broadly used in the various
field namely: ecology, field of forensic science, field of wildlife and
Ethology for the hair identification of species of interest. By
using this hair identification key it can be easier and suitable to identify
and confirm the species largely to know feeding ecology of carnivore animals in
both protected and non-protected areas. It can be easily applicable for hair
identification in case of human-carnivore conflict study.
ACKNOWLEDGEMENTS-
Authors are grateful to Dr. A. P. Singh,
Chief Conservator of Forest, Junagadh for his encouragement and supports for
the study. Our sincerely thank to the laboratory officials and technicians like
Mr. V. O. Yadav, Mr. M. S. Chand, and Mr. M. Joshi for their help during the
laboratory investigation of sample specimens.
CONTRIBUTION
OF AUTHORS-
Research concept- Vibhuti B. Raval
Research design- Vibhuti B. Raval
Supervision- Ashvinkumar H. Italiya
Materials- Ashvinkumar H. Italiya
Data collection and processing- Vibhuti B. Raval
Data analysis and interpretation- Vibhuti B. Raval
Literature search- Vibhuti B. Raval
Writing article- Vibhuti B. Raval
Critical review- Bhupat B. Radadia
Article editing- Vibhuti B. Raval
Final approval- Bhupat B. Radadia
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